AtARF1 bound to IR8 with a K d of 140 nM, a value 16-fold lower than the K d derived from ARF5, and to DR5 with a K d of 570 nM, a value 10-fold weaker (Fig. 6B). Fig. 6. Single-molecule FRET assays reveal differential ARF-DNA affinities. (A) Cartoon describing the single-molecule FRET assay. IR8- or DR5-containing (arrows) ds oligonucleotide ... WebJan 23, 2007 · AtARF1 1 publication. Alternative names. Protein BFA-VISUALIZED EXOCYTIC TRAFFICKING DEFECTIVE 1 1 publication. Gene names. Name. ARF1 1 publication. Synonyms. ARF1A1C 1 publication, BEX1 1 publication. ORF names. T3D7.2 Imported, T8I13.1 Imported. Ordered locus names. At2g47170 Imported. Organism names.
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WebWe next asked whether dimer stability is a more generic parameter defining DNA binding affinity across the ARF protein family. To this end, we purified the DNA-binding domains … WebSep 27, 2016 · Expression of AtARF1 gene. G, Expression of AtCullin gene. For genes expression, actin gene was utilised as an inside handle. Mistake bars, mean6SD (3 impartial organic replications)with sixteen/eight h working day/evening in a growth chamber. HSV inhibitor September 27, 2016 Uncategorized dallas roosevelt high school football roster
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WebJan 14, 2015 · Simultaneous expression of MSD1-YFP, the plastid marker WxTP–DsRed, and either AtARF1(T31N), AtARF1(Q71L), or AtSAR1(H74L) indicated that the plastid targeting of MSD1-YFP was inhibited in cells expressing the ARF1 and SAR1 mutant proteins (Figure 5d,f). The overall results clearly indicate that MSD1 is a multilocalizing … WebMay 29, 2024 · Taking this advantage, we identify a possible candidate gene, HcARF5, a homologous of AtARF1. β-ocimene (acyclic monoterpene) is the main floral volatile compounds released from the H. coronarium flowers, playing numerous biological functions in plants especially attract pollinators and mediate defense response to herbivory (Abbas … birchview home birch hills