2024 C-myc epitope tag antibody

C-myc epitope tag antibody

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August undefined, 2024

WebJan 28, 2024 · We tested by Western blotting six antibodies that recognize the c-Myc epitope tag, including monoclonal antibodies 9E10, 4A6, 9B11, and 71D10 and …

Anti-Myc tag antibody (ab152146) Abcam

WebSep 27, 2024 · Epitope tags are widely used in various applications, but often lack versatility. ... Ramsay, G. & Bishop, J. M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product ... WebEpitope tags such as V5, c-myc, and HA are biological structures, usually proteins, that are genetically engineered onto either the N or C terminus of a recombinant protein. Tags function as universal epitopes, easily … for rent chesapeake va 23321

Monoclonal Antibodies Antibodies.com

WebWhether you’re studying endogenous c-Myc or Myc-tagged proteins, aim for perfection with our highly validated Myc antibodies. Mouse monoclonal antibody (9E10 clone) to the Myc tag to study Myc-tagged proteins. Rabbit monoclonal antibody (Y69) to c-Myc to specifically detect endogenous c-Myc. Both antibodies are: Rated as excellent by users ... WebMyc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™-Mouse Monoclonal Antibody Achetez Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™ WebPurified anti-c-Myc Antibody - The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. ... (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ ... digital and cyber literacy

In vitro evolution of myc- tag antibodies: in-depth …

MYC Epitope Tag Antibody (600-401-381) Rockland

WebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. WebAnti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the … digital and app innovation microsoftWebMar 22, 2024 · One of the most widely used epitope tags is the myc-tag, recognized by the anti-c-Myc hybridoma antibody Myc1-9E10. Combining error-prone PCR, DNA … digital and cloud services

"WebProduct Details. Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species. This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives. The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions. " - C-myc epitope tag antibody

C-myc epitope tag antibody

WebThe anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are … WebImmunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.

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WebCatalog-Num Product Name Quantity Price Supplier Category; Catalog-Num Product Name Quantity Price Supplier; NBN Monoclonal Antibody [3E4] (100 ug) 569-Eur: EpigenTek: … WebCatalog-Num Product Name Quantity Price Supplier Category; Catalog-Num Product Name Quantity Price Supplier; NBN Monoclonal Antibody [3E4] (100 ug) 569-Eur: EpigenTek: RET Monoclo

WebBy Stewart Newlove, PhD. Epitope tags are short peptide sequences, generally composed of 10-15 amino acids, designed to create a molecular handle for a protein. An epitope tag can be placed anywhere within a protein but typically are placed on either the C-terminus or N-terminus in order to minimise tertiary structure disruptions that may alter ... WebBioLegend's Posi-Tag Epitope Tag Protein can be utilized as a positive control in Western blotting experiments where many epitope tags are commonly used. Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His, and has an approximate molecular weight of 45 kDa.

WebResults for c-myc antibodies 447 products found ... (House mouse), and Epitope tag/Fusion protein. Recombinant Anti-c-Myc (phospho T58) antibody [EPR17923] ab185655 is a primary antibody from Abcam. 18 Citations Host: Rabbit Clonality: Recombinant (EPR17923) Validations: None Available WebWe offer a wide range of validated antibodies for epitope tags. ️ High Quality Products ️ Low Prices ️ 100% Guarantee. Useful Links. 01223 298 875; ... Mouse monoclonal [MYC699] antibody to c-Myc. Applications: ELISA, Flow Cytometry, IF: Reactivity: Human, Chimpanzee, Gorilla: Conjugate: Unconjugated: View Product. Anti-RFP Tag Antibody ...

WebWe offer a wide range of validated antibodies for epitope tags. ️ High Quality Products ️ Low Prices ️ 100% Guarantee. Useful Links. 01223 298 875; ... Mouse monoclonal …

WebThe Myc tag, derived from the human c-myc gene, is a peptide epitope tag used in detection or purification of recombinant proteins. The 10-residue peptide sequence consists of EQKLISEEDL and has a mass of approximately 1202 Da. It can be fused to the N- or C- terminal end of a protein, but placement directly behind of the signal peptide of secreted … digital and ict oversight frameworkWebSpecificity / Sensitivity. Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in … digital and connected worldWebMyc Tag Antibodies. The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C … Find the Myc Tag antibody that fits your needs. Choose from 1 of 77 Myc Tag … for rent chesapeake vaWeb(B) 9E10 antibody, which specifically recognizes the human myc epitope tag and does not cross-react with murine c-myc, was used to immunoprecipitate myc-tagged ΔN89β-catenin from extracts of the ... digital and content marketingWebWhat are epitope tags? Antibodies are an essential tool to determine the localization and expression of target proteins, and for purification in the presence of numerous other proteins in living organisms. ... c-Myc protein is a transcription factor involved in the cell cycle and apoptosis. Abnormalities in c-Myc, such as mutations and ... digital and electronic communicationWebJan 28, 2024 · All three rabbit antibodies showed a preference for the Myc epitope tag in a C-terminal position (in the contexts of vectors pCMV-Myc C and pcDNA3.1/Myc). In particular, antibody 9106 (Abcam) displayed a strong bias for the C-terminal tag position, with the pCMV-Myc C context protein about eight times better detected than the pCMV … digital and information literacy frameworkWebMyc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™-Mouse Monoclonal Antibody Achetez Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™ for rent chester il