WebKeep the RNA solution on ice to reduce RNA hydrolysis. 4. Set up the following annealing reaction to produce a 20 µ m solution of siRNA duplex: 5. Incubate for 5 min at 95°C, and then for 2 h at 37°C. Store the siRNA solution at −20°C or −80°C. Annealed siRNA duplexes remain stable at least for 6 mo at −20°C and for years at −80°C. WebEarlier calorimetric studies had indicated that despite the absence of a GpC sequence, the self-complementary octamer d(CGTCGACG) binds strongly to actinomycin D ...
G-quadruplex DNA: a novel target for drug design - PubMed
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דופלקס (Duplex) - מדריך התרופות שירותי בריאות כללית
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