WebThe transcribing cellular polymerase ignores the polyA/stop signal which is present 300bp away from the start, but the same site is efficiently recognized when the RNA is 3.5kb. Possibly the host RNA-polymerase II is in an activated state during transcription of the first hundreds bp. This would prevent pausing and termination factors function ... WebJul 2, 2024 · The present work aims to characterize the development of the Delphi method, based on scientific indicators related to publications with high quality indexes. As a result of an automated search in Scopus, a database of 10341 documents recorded since 1969, has been formed. Among the main results, it is observed an exponential growth of the …
What is the best polyA signal for mammalian gene expression?
WebFor general laboratory use. Shipping: shipped on gel packs Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Store dark. WebApr 7, 2024 · The qPCR was performed in Light Cycler 480® (Roche) using SYBR Green detection reagent (04707516001, Roche). Digital PCR was performed in the QX200 AutoDG Droplet Digital PCR system (Bio-Rad) using the detection reagent kit for probes (1863024, Bio-Rad). In both systems primers for BGH polyA sequence (Fw: 5′- … colonial plating richmond va
Evaluation of two main RNA-seq approaches for gene ... - Nature
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