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WebSep 1, 2005 · The tetracycline transactivator gene (tTA off) was knocked-in to the Pdx1 locus such that the tTA gene is expressed under the control of the endogenous Pdx1 promoter (Pdx1 tTA mice). Mice carrying a transgene encoding the Pdx1 gene (Tg Pdx1 ) and a reporter gene (enhanced green fluorescence protein [EGFP] under the control of a … Web总结一下tTA*的作用,tTA*使得神经元在不再兴奋时,甚至四环素存在时,依旧持续地使目的基因蛋白表达。一朝开启,永世表达。 这就是Teg-off系统。强力霉素存在时,Tet标记系统关闭。强力霉素缺席(off)时,Tet标记系统开启,兴奋神经元开始表达荧光蛋白。 tow hitch recall
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